Abstract:In this article,some researchs on the karyotypes of the massion pines from nine geographical seed sources and analysis on the asymmetric coefficient of the karyotypes were made,the results showed that:massion pines' Karyotypes are all 2n = 24 = 24m,a metacentric chromosome,belong to a relative original group"IA";some relatively symmetric karyotypes were owned by the pines whose provenance such as Qianshan locate in the centric region of oistribution,on the contray,some relatively asymmetric karyotypes were obtained by the pines whose provenance such as Shaxian,Nanzheng locate in the marginal area of distribution iso,a siginificant geographical distribution rule (or difference)among the different provenance's pines exist for their karyotypes.