Abstract:Pollen of seed orchard of Cunninghamia lanceolata on 18 years old as explant. Culture medium made of 9 manages which as different concentration of sucrose and pH on pollen of germination test. And then observe the germination of pollen in low microscope on after 24~36 hours of inoculation. In this test was found that pollen of germination was different in different culture medium. The best culture medium is that concentration of sucrose is 20% and pH is 5. 5 in germination of pollen of Cunninghamia lanceolata.