杉木采样时间及DNA提取方法的研究

Study of sampling time and means of DNA extracting of cunninghamina lanceolata

摘要:

从材料中提取ＤＮＡ时，必须因材而异，杉木材料中含有较多的酚类等粘性物质。对杉木的采样时期及其 ＤＮＡ提取方法进行的研究表明：５月份采取杉木新芽 ３ ｇ，用 ＣＴＡＢ洗净法可以获得浓度和纯度最高的ＤＮＡ。

Abstract:

The means of extracting DNA are different from research materials. Study of sampling time and means of extracting DNA of cunninghamia lanceolata have been discussed in this paper. The results show that the highest concentration and purity DNA can be obtained when sampling in May and by the means of CTAB washing.