利用RCR-RAPD法和同功酶分析法日本柳杉无性系的识别方法研究

Study of ldentifying Clones of Cryptomeria japonica By the Means of PCR-RAPD and lsozyme Analysis

摘要:

以日本福岛县林业试验场日本柳杉种子园的 ２４个无性系为材料，利用 ＰＣＲ—ＲＡＰＤ法和同功酶分析法对日本柳杉无性系的识别方法进行了比较研究。结果显示：同功酶法和由７个标记构成的ＲＡＰＤ分析法不能完全识别２４个无性系，用１７个和２７个标记构成的ＲＡＰＤ分析法识别这２４个无性系是可能的；同功酶标记是一种基因型标记，具有稳定性高的特点，适合于分析遗传构造的相似程度，特别适合于群体遗传分析，但是，由于能获得清晰话带的同功酶种类有限，在用于个体识别上比较困难；ＲＡＰＤ标记是一种显性标记，稳定性较差，但只要反应体系和条件稳定，利用ＲＡＰＤ标记正确进行无性系识别是完全可能的。

Abstract:

To identify clones of Cryptomeria japonica the Comperation of PCR-RAPD and isozyme anaysis is conducted. With the material of 24 clones from seedling garden-of Fukushima forestry experiment Station in Japan. The results show that isozyme and RAPD with 7 markers cannot identity 24 clones Completely,but RAPD with 17 markers and 27 markers probably identify these 24 clones. The author also consider that isoxyme marker is a genetic marker which exhibits very good reproducibility. So it's fit for genetic structural samility analysis,especially for group genetic analysis,but difficult for individual identifying because the types of isozyme with clear bands is limited. PAPD is a dominant marker which is often unstable,but if Can identify clones corretly with the stable reaction system and condition.