半夏不同采收期总生物碱含量的动态变化研究

Research on Dynamic Change of Total Alkaloid Content in Pinellia Ternate (Thunb.) Breit.during Different Harvest Period

摘要:

采用氯仿提取 ,依据酸性染料比色法的原理 ,在 41 7nm波长下测定半夏中总生物碱含量 ,进行方差分析。结果表明 :获得良好的线性关系 (r=0 9989)和回收率 ( 1 0 0 1 %) ,RSD为2 3%。半夏不同采收期总生物碱含量差异显著 (F =2 31 3,P <0 0 5 )。结论 :测定方法简单快速 ;半夏不同采收期总生物碱含量以 8月中下旬最高 ,每株平均总生物碱产量以 1 0月中下旬至 1 1月上旬最高 ,半夏最佳采收期以 1 0月中下旬至 1 1月上旬为宜

Abstract:

The total alkaloid content in Pinellia ternate has also been measured out under 417 nm of wavelength according to principle of color method by acidic dye through extraction by chloroform and a variance analysis has been carried out. The result showed that there can obtain a curve with good linear relationship ( r =0.9989), high recovery rate (100.1%) and 2.3% of RSD and there also exists significant difference in total alkaloid content ( F=2.313,P<0.05 ) among different harvest periods, we can make such conclusions that this method is simple and quick, total alkaloids content reaches the highest point in the last ten days of August, but total average alkaloids output per plant is the highest in the period from middle and last ten day period of October to first tendency of November and the same is for optimum harvest period.