Abstract:In order to study requirement for nitrogen nutrition by seedling of Metasequoia glyptostroboides,a trial study was conducted according to six application levels of 0.0, 1.0,2.0,3.0,4.0 and 5.0 g nitrogen/pot, the result showed that: 1. seedling height, ground diameter, branching number and its total length raise along with increment in nitrogen application rate, however, these indexes fall down along with the continous increment in nitrogen application rate after excess of 3.0 g/15 kg soil in nitrogen application rate,reaction to nitrogen is similar to these by content of chlorophyll in seedling of Metasequoia glyptostroboides.2. The contents of nitrogen in root, stem and leaf rise along with increment in nitrogen-application rate.