Two populations of Liriodendron in Pingbaying Forest Farm of Xianfeng county in Enshi prefecture and Yuguan town of Wufeng County of Yichang prefecture were surveyed in this paper, and genetic variability of the two stands was evaluated using isozyme techniques and its genetic diversity was discussed. The proportion of polymorphic loci both at 99% and 95% criteria in Xianfeng populations were 57.10%. The corresponding in Wufeng population were 42.86% and g8. 57% respectively, The inbreeding coefficients in Xianfeng and Wufeng were nearly zero, namely -0.027 and 0. 007 respectively.