一种提取刺槐叶片DNA的有效方法

Effective Way on Isolation of DNA from Leaf Extracted of Robinia Pseudoacacia

摘要:

以刺槐叶片为材料,摸索出了一套改良的SDS法.与传统方法相比,该方法DNA收取量大,操作简单、纯度高、完整性好,适用于遗传工程的DNA制备.

Abstract:

A procedure for isolation of plant total DNA from leaf of Robinia pseudoacacia with a modified SDS method is introduced. Comparing to the traditional methods, it is simple to operate, higher content and good integrity of DNA. It is suitable to prepare plant DNA for the genetic engineering,