东方百合组培快繁及试管苗健化栽培技术研究

Study on Cultivation Technique in Fast Propagation of Tissue Culture and Test-tube Seedling Exuberance for Eastern Lilium Brownii

摘要:

以东方百合中市场占有率高的3个优良品种的鳞茎作为外植体进行组培快繁,对组织材料快速繁殖条件优化的研究表明,生长调节剂组合对百合愈伤组织分化和小鳞茎增殖有较大的影响,东方百合鳞茎基部近基盘处是进行芽诱导培养的极好外植体.东方百合试管苗出瓶后必须进行试管苗健化栽培,这个阶段是百合商品球能否达标的关键.以东方百合品种西伯利亚为材料,从试管苗出瓶后栽培基质、水分管理、施肥方式等关键因素入手,探索了东方百合试管苗健化期最佳的管理模式,使其成活率达到了98%以上.

Abstract:

Take bulb of three excellent varieties occupying high ratio in market of eastern Lilium brownii as test material. The research on optimum condition of the fast propagation of culture material shows that the combination of growth regulator had larger effect for differentiation of callus of Lilium brownii and proliferation of small bulb. The basic bulb of eastern Lilium brownii near the basic tray was a better ex-plant to carry out the induced culture of primary bud. The exuberance cultivation of the trial-tube seedling of eastern Lilium brownii must be made after the seedling come out from bottle, this stage was a key point for the commercial ball of Lilium brownii that can reach the standard or not. Take variety of eastern Lilium brownil of Siberia as materials, explores the optimum management model during the exuberance stage in breeding the seedling that the survival rate reaches over 98% after the seedling lifts from the bottle by taking key measures in cultural medium, water management and fertilizer etc.