Abstract:The authors have studied genetic structure of natural populations of Sassafras tzumu Hemsl. using isozyme analysis. The results showed the proportion of polymorphic loci, average number of alleles per locus, and average expected heterozygosity were 49.5%, 1.649, and 0. 280 in three populations, respectively. The Frs values indicated predominant excesses of homozygotes and deficiencies of heterozygotes were present within the populations, which were in unblance condition.