菊花脱除花叶病毒组培技术研究

Study on Technique of Tissue Culture of Virus B Free Chrysanthemum Morifolium

摘要:

以患花叶病菊花0.5～0.8 mm幼嫩茎尖为外植体,愈伤组织的诱导在MS BA2.0mg/L NAA0.1 mg/L培养基上效果最好,诱导率达96.0%;愈伤组织分化出芽以MS BA3.0 mg/L NAA0.1 mg/L效果最佳,分化率达60.0%,每块愈伤组织平均分化出4个芽;幼苗在1/2MS NAA0.1 mg/L培养基中生根率达92.0%,株平均生根5.1条.组培苗经症状观察和汁液传染实验试验检测,以后种方法结果为依据,获得菊花脱毒苗19株,脱毒率为63.3%.

Abstract:

The shoot tips with a length of 0.5~0.8 mm is suitable for explants,which came from Chrysanthemum with CMV.Callus grew well on MS+BA2.0 mg/L+NAA0.1 mg/L and 96.0% explants formed.About 60.0% callus developed with the best result on MS+BA3.0 mg/L+NAA0.1 mg/L,and there were 4 buds every callus.The buds were cut and transferred into 1/2MS+NAA0.1 mg/L,and the rooting rate was 92.0% with 5.1 roots per average plant.Two ways about symptom observing and sap transmission were used in the experiment to test virus free seedlings. By testing,there were 19 Chrysanthemum virus-free seedlings,which virus-free rate was 63.3% through the second method.