桉树外植体诱导褐变原因初探

Initial Discussion on Reason of Induction Brown - turning outside Eucalyptus Plant Body

摘要:

桉树外植体诱导产生褐变的因子很多,在此只讨论不同无性系、不同木质化程度的茎段、不同的消毒处理、不同的光照对外植体褐变的影响。从试验结果得出:不同的无性系外植体产生褐变程度不同,采集半木质化或刚木质化的茎段,用0.1%HgCl2分两步消毒可以减轻褐变,在培养过程中采用全暗光的方法有效控制褐变,萌芽率也高。

Abstract:

There are many factors causing brown-turning on induction outside Eucalyptus plant body, here, only discusses different clone, different stem section of lignifications degree, different disinfection processing and influence of different illumination to brown-turning outside plant body. The result is obtained from the test that different clone outside plant body to cause different brown-turning degree, gather stem section of half or just lignifications to reduce brown-turning using 0.1%HgCl2 to disinfect by two steps, adopts method of entire obscure ray to control effectively and germinating rate is also high during breeding process.