杨树亲-子代叶片的过氧化物酶同工酶分析

Study on Peroxidase Isozyme of Leaves between Parents and Progenies of Populus L.

摘要:

通过聚丙烯酰胺不连续垂直板凝胶电泳,对杨树6个全同胞家系的亲本及子代叶片的过氧化物酶(POD)同工酶,及其与生长量和杂种优势之间的关系进行了研究.结果表明:供试单株及不同家系在酶谱带数、迁移率、酶的活性强弱等方面存在一定的差异,即使同一家系内亲代与子代之间也存在差异,子代主要表现为双亲互补型酶带.POD同工酶分析可用于杨树亲缘关系的鉴定.子代与母本的相似系数比子代与父本的相似系数高,偏向母性遗传.同时,研究结果还显示过氧化物酶酶带数和酶强度与地径生长量之间均呈显著性正相关,相关系数分别为0.89和0.83;酶带数和酶强度与地径超亲优势之间也呈显著性正相关,相关系数分别为0.88和0.84;而酶带数和酶强度与苗高生长量及苗高超亲优势之间的相关性均不显著.酶带的谱型与生长量和杂种优势之间也没有表现出明显的相关性.因此,仅从过氧化物酶同工酶酶谱的带数、强弱和谱型来判断杨树的生长量和杂种优势是不完全合理的.

Abstract:

The peroxidase isozyme of leaves and its relationship with growth and heterosis of 6 Populus L.full-sib families were determined by means of polyacrylamide gel electrophoresis.The results indicated that the tested individuals and families differed significantly in number,Rf,activity or width of the enzyme bands,even though some of them within a same family.Band type of progenies were mainly parental complementary.Therefore,POD isozyme can be used to identify their inheritable relationships.Our results also ...