Abstract:The effects of continuously planting on soil biological characteristics of Populus L.plantation were studied in Jianghan plain by using space for time method.Comparing to first generation forest,The result showed that the second generation forest quantities of fungi,actinomycial,bacteria and the total microbe numbers decreased by 34.38%,14.38% 56.63% and 56.05% respectively;and the activities of soil phosphataese,urease,sucrase,protease,catalase decreased by 20.00%,11.54%,29.39%,50.00%,1.92% respectivly,whi...