杂交马褂木组织培养技术研究

Study on the Tissue Culture Technique of Liriodendron chinense × L．tulipifera

摘要:

摘要：外植体采自杂交马褂木的幼树或扦插苗，采集部位为其顶芽或腋芽，最佳培养基为MS+BA 2.0 mg/L +NAA 0.1 mg/L。采用DCR为基本培养基，加入抗褐化试剂柠檬酸50.0 mg/L，Vc 50.0 mg/L，继代增殖率可以达到2.8倍，最高达到4倍。生根培养基为1/2MS+NAA 1.5 mg/L +活性炭0.5 g/L，瓶苗生根率高达61%。组培苗直接移栽到红心土，成活率达到93%以上。

Abstract:

Abstracts:The explants were taken from the top buds or axillary buds of the young trees or cuttingpropagated seedlings of Liriodendron chinense ×L．tulipifera. The optimal medium for the explant induction is MS+BA 2.0 mg/L +NAA 0.1 mg/L. The subculture medium was DCR+BA 2.0 mg/L +NAA 0.05 mg/L +citric acid 50.0 mg/L +Vc 50.0 mg/L, the proliferation rate could reached 2.8, and the highest proliferation rate was 4. The rooting medium was 1/2MS+NAA 1.5 mg/L +activated charcoal 0.5 g/L, and the rooting rate in the bottle could reach 61%. The tissuecultured plantlets were transferred into the red core soil, and the survival rate was over 93%.