组培苗百合种质资源ISSR分析

The ISSR Analysis on Germ Plasm Resources of Lilium browniifrom Tissue Culture Seedling

摘要:

摘要：本研究应用ISSR分子标记对日本百合、荷兰百合以及湖北野生百合共34份组培苗材料进行了遗传多样性分析。从40条引物中筛选出4条重复性好，条带清晰的引物进行PCR扩增，共扩增出多态性条带76条。供试百合材料的观察等位基因数（Na）为 2，有效等位基因数（Ne）为 1.2623，Nei's (1973)基因多样性指数（H）为0.196 3，Shannon 信息指数(I)为0.338 8。湖北野生百合遗传多样性较高，日本百合和荷兰百合相对较低。根据标记分析结果，基于供试样品间遗传距离，采用UPGMA分析方法构建了聚类图，对不同来源百合样品的亲缘关系进行了初步分析。

Abstract:

Abstracts:The germ plasm resources of 34 tissuecultured seedling individuals from Lilium japonicum，Lilium hybrids and Lilium brownii of Hubei were identified using intersimple sequence repeat (ISSR) markers。4 primers were screened out from 40 ISSR for PCR and 76 polymorphism sites were amplified out totally。For Lily Tissuecultured seedling, the average number of alleles (Na) per locus was 2, and the effective number of alleles (Ne) was 1.2623. The Nei's gene diversity (H) and Shannon's information index (I) were 0.1963 and 0.3388, respectively. The genetic diversity of Lilium brownii was much higher than that of Lilium japonicum and Lilium hybrids.UPGMA cluster analysis based on ISSR data was used to construct the dendrogram.The preliminary proof proved that it was feasible to analyze the system evolution and genetic relationship of Lily plantsspecies by using ISSR．