邓恩桉茎段组织培养试验初探

Primary study on tissue culture of stem segment of Eucalypts dunnii

摘要:

摘要：采集邓恩桉茎段进行组织培养。试验结果表明：半木质化或刚木质化的茎段用浓度为0.1%Hgcl2消毒6min，用无菌水冲洗3~4次，再用Hgcl2消毒2 min效果最好，杀伤率低，获得率高达67.5%；芽分化及增殖培养基用改良MS+3%糖+3%的琼脂+VC0.01 mg∕L+VB20.015 mg∕L+BA0.5 mg/L+NAA0.25 mg/L，增殖系数达4倍，芽生长正常，不产生“玻璃化”苗；在IBA0.5 mg/L+ABT0.25 mg/L及IBA0.5 mg/L+NAA0.5 mg/L两种激素组合对邓恩桉不定根诱导有一点作用，但效果不理想，生根率只有20%，愈伤头大，上杯不容易成活。

Abstract:

Abstracts:Stem segments of Eucalypts dunnii were used for tissue culture in this paper. Best effect with low killing rate and up to 67.5% gain rate was obtained according to the following procedures. Firstly, semi-lignified or lignified stem segments were disinfected for 6 minutes with 0.1% Hgcl2, washed 3-4 times with sterile water, then disinfected with Hgcl2 for 2 minutes. Modified medium with MS +3% sugar +3% agar +VC 0.01 mg/L +VB20.015mg/L + BA0.5mg/L +NAA0.25mg/L was used for bud differentiation and proliferation. The multiplication coefficient was 4 times, and buds grew normally with no vitrification. The two hormone combination of 0.5mg/L IBA +0.25mg/L ABT and 0.5mg/L IBA +0.5mg/L NAA had some effects on root induction of Eucalypts dunnii.But the effects were not very good. The rooting rate was only 20% with big callus, and goblet cell didn't survive easily.