I-69杨与小叶杨杂交种胚培养研究

Researches on Embryo Culture of Hybrids Between Populus deltoides cv.“Lux” and Populus Simonii

摘要:

摘要：本研究利用组织培养技术对进行培养挽救，以为了提高育种成功率。试验结果表明：外植体的最佳消毒方法为75%酒精消毒30 s后在01%升汞处理8 min，幼胚的成活率为90%。随着外植体消毒时间增加，幼胚成活率先上升后下降。幼胚最佳萌发培养基为1/2MS +BA0.1 mg·L-1+NAA0.01 mg·L-1。最佳继代增殖培养基为MS+ BA0.1 mg·L-1+NAA0.01 mg·L-1，增殖系数达到633。对不定芽增殖最有利条件为：pH值6.0、琼脂浓度6 g·L-1和蔗糖浓度30 g·L-1。最佳生根培养基为 1/2MS+IBA0.2 mg·L-1，生根率为93.2%。

Abstract:

Abstracts:In this study, tissue culture technology was used to rescue immature embryo of hybrid from artificial crossing between Populus deltoides cv.“Lux”and Populus simonii in order to improve breeding efficiency.The study showed that the optimal sterilizing methods of explants were dipping to 75% Ethanol for 30 s followed by treating with 0.1% HgCｌ2 for 8 min，and the survival rate of immature embryo was 90%.With increased time in explants sterilization, embryo survival rate increased and then decreased. 1/2 MS+NAA0.01 mg·L-1+BA0.1 mg·L-1 was optimal germination medium of immature embryo.MS+NAA 0.01 mg·L-1+BA0.1 mg·L-1were optimal proliferation medium. The Proliferation coefficient was 6.33. The optimum culture medium for proliferation contained 30 g·L-1sucrose，pH6.0，6 g·L-1 argar. 1/2MS+IBA0.2 mg·L-1 were the optimum rooting medium, and the rooting percentage was 93.2%.