湖北油茶种质资源SSR分析

Genetic Analysis of the Germ Plasm of Camellia OleiferafromHubei Province Based on SSR Marke

摘要:

摘要：利用油茶SSR标记对湖北省的油茶种质资源进行分析，结果表明：10个不同SSR位点所揭示的86份油茶种质间存在较丰富的遗传多样性，平均观察等位基因数为4.9个，平均有效等位基因数为3.2个,平均Shannon 信息指数为1.13，平均杂合度为0.25。36个优良品种的聚类分析结果表明：同一地区的油茶品种相对外地品种具有较近的亲缘关系，且各品种间存在一定的差异，可以利用SSR标记进行品种鉴别。遗传参数受抽样群体大小影响的分析表明：湖北油茶资源的有效等位基因数（Ne）和基因多样度（h）受抽样个体数的影响很大，当样本数达到50时，Ne和h值趋于稳定。

Abstract:

Abstract:The genetic analysis of the germ plasm of Camellia oleifera from hubei province based on SSR marker has been done in this study. The result showed that the relatively rich genetic diversity existed in 86 germ plasms of Camellia oleifera revealed by 10 different SSR loci, and the average observed number of alleles was 4.9, the average effective number of alleles was 3.2, the average shannon＇s information index was 1.13, the average heterozygosity was 0.25. The result of cluster analysis of 36 varieties indicated that the varieties from same area had a closer relationship than those from other areas, and difference existed between different varieties and the SSR marker could be used for species identification. The effect that sampling size influenced the genetic parameters showed that the effective number of alleles（Ne）and genetic diversity (h)of Camellia oleifera from hubei province were critically affected by the number of sampled individuals, and when the number of samples reached 50, the Ne and h would appear stable values.