秋石斛组织培养的研究

Tissue Culture Technique of Dendrobium hybrida

摘要:

摘要：秋石斛为兰科植物，具有较高的观赏价值。本试验旨在建立秋石斛的工厂化育苗模式。采用秋石斛的高位芽为外植体，用75%酒精消毒1 min后，再用0.1% HgCl２消毒20 min，达到最佳的消毒效果。试验发现最佳的不定芽诱导培养基为MS+6-BA1.0 mg·L-1+IBA 0.5 mg·L-1+ 土豆泥80 g·L-1+活性炭1 g·L-1，增殖系数为4.5。最佳的生根壮苗培养基为MS + IBA0.8 mg·L-1+ 土豆泥80 g·L-1+ 活性炭1 g·L-1，生根率99%。移栽成活率达到95%。

Abstract:

Abstracts:Dendrobium hybrida belongs to family Orchidaceae with high ornamental value.We established a mass production method for D. hybrida.Taking high position buds as the explants,the optimal disinfection treatment was 75% alcohol for 1 minute and then 0.1% HgCl2 for 20 minutes.The results showed the optimal medium for proliferation of adventitious bud was the MS medium supplemented with 1 mg·L-16-BA,0.5mg·L-1IBA,100 mg·L-1 mashed potato and 1 mg·L-1 activated charcoal (AC),providing a proliferation coefficient of 4.5. The optimal medium for rooting was MS medium supplemented with 0.8 mg·L-1IBA,100 mg·L-1 mashed Potato and 1 mg·L-1AC, providing 99% rooting rate and 95% survival rate.