白僵菌生产中发酵罐培养技术研究

Technology Research of Liquid Fermentation Tank Culture of Beauveria bassiana

摘要:

摘要：为掌握白僵菌发酵罐培养技术，白僵菌可工业化生产，以宜昌夷陵区白僵菌菌种为材料，对白僵菌液体发酵罐发酵培养的培养基、菌种生长形态、生长环境的pH值进行试验研究，从而确定转罐时间等液体发酵罐发酵技术参数。结果表明：在液体发酵培养中，碳、氮、磷为白僵菌生长必需的三要素，不同生长时期三要素比例不同，初期加入少量微量元素有助于提高白僵菌孢子生长速率和增加数量；在白僵菌液体发酵生长史中，初始pH值为6，继而在pH值4～5的环境中生长速率最快；前42～45 h在种子罐中主要产菌丝体，在转罐后21～26 h发酵罐中主要产孢子体。

Abstract:

Abstracts:In order to master the technology of fermentation culture of Beauveria bassiana, and put the culture of expanding Beauveria bassiana into industrial production, we used Beauveria bassiana strain of Yiling district,Yichang as the material and fermentation technical parameters such as the appropriate liquid fermentation medium and the growth morphology of strain and pH values of growth environment were studied by liquid fermentation tank culture which could help identify the time of transferring liquid from seed tank to fermentation tank. The results showed that Carbon, Nitrogen and Phosphorus were three kinds of necessary nutrient elements in the liquid fermentation. Besides,these three essential factors for the growth of Beauveria bassiana had different proportion,the addition of a small amount of microelement in the liquid fermentation medium at the early stage could increase growth rate and the number of Beauveria bassiana evidently;Beauveria bassiana has the cyclic growth history that is mutual transformation between saprophyte and mycelium in its multistage continuous liquid fermentation,and the comparative analysis of spore form and the numbers showed that the fastest growth appeared in the pH value between 4 and 5 which the growth environment of Beauveria bassiana owned by self regulation and artificial adjustment,although we had given condition that the medium was a pH of 6 initially;Considering the growth morphology and the number and the activity of Beauveria bassiana and the workshop operation schedule,we made the following arrangements,and the first process was producing mycelium in the seed tank for 42～45 hours, and the second one was producing saprophyte which was the strain of solid fermentation production in the fermentation tank for 21 to 26 hours.