Abstract:Biofilmed biofertilizers(BFBF)are different from regular biofertilizers.Different plants symbiose with different microorganisms,therefore BFBF should be tested before they are used in agriculture and forestry.In the present study,a new BFBF was used in culture of Pinus massonicana seedlings.These results showed that the BFBF significantly increased net photosynthesis rate and decreased intercellular CO2 concentration,and at the same time significantly reduced the use efficiency of water and light.The BFBF significantly increased Y(II),ETR,and Fv/Fm,but showed no effect on qP and qN.Increase in net photosynthesis rate induced by the BFBF resulted in significant increase in root biomass, shoot biomass,and total biomass.