赛黑桦组织培养技术研究

study on Tissue culture of Betula schmidtii

摘要:

以赛黑桦休眠枝萌发的腋芽为外植体,研究了不同种类和不同浓度植物激素对赛黑桦外植体诱导、愈伤组织诱导、 不定芽增殖和生根等环节的影响。结果表明:①外植体芽的诱导培养以WPM + 6-BA0. 5mg ● L- 1 + NAA0. 1mg ● L-1为最 佳培养基 ; ②愈伤组织诱导以 WPM + 6-BA1. 0 mg ● L- 1 + NAA 0. 02mg ● L- 1 为最佳培养基;③在芽增殖培养中以 WPM+ 6-BA0. 1 mg ● L- 1 + NAA 0. 02mg ● L-1增殖效果最好,增殖系数达4. 0;④生根培养以 WPM+ NAA0. 05mg ● L- 1 效果最佳,生根率 91. 7%;⑤生根苗移栽成活率达 90%以上

Abstract:

The axillary buds sprouting from dormant shoots were used as explants to study the effects of plant hormones on explant induction, callus induction, adventitious proliferation and rooting. Results showed that : ①The medium of WPM十6-BA 0. 5 mg ● L—1十NAA 0. 1 mg ● L—1was the best initiation culture medium: ②The medium of WPM十6-BA 1. 0 mg ● L—1十NAA 0. 02mg ● L—1 was the best callus induction medium . ③The medium of WPM十6-BA 0. 1 mg ● L—1 十 NAA0. 02mg ● L—1 with propagation coefficient 4. 0 was the best proliferation culture medium . ④The medium of WPM十NAA 0. 05mg ● L—1 with rooting rate 91. 7% was the best rooting culture medium . ⑤The survival rate of trans- plants was higher than 90% .