In order to more rationally and scientifically protect the biodiversity of the three national nature reserves in southwestern Hubei , this article combines field investigations and document analysis , and refers to the evaluation methods of the“Regional Biodiversity Evaluation standards ”to evaluate three The status of biodiversity in national nature re- serves . The results showed that:①xingdou Mountain Nature Reserve》s biodiversity index was 42. 87, Qizimei Mountain Nature Reserve was 44. 25, Mulinzi Nature Reserve was 49. 17, and the biodiversity status was“medium”;②Mulinzi com- pared with xingdoushan Nature Reserve and Qizimei Mountain Nature Reserve , Mulinzi Nature Reserve has more ecosys- tem types and richer biodiversity;③There are 415 threatened species in 3 nature reserves , including 231 plants . species , 184species of animals , a wide variety of species , need further protection .