Abstract:Sequoia sempervirens is an important tree species for landscaping and timber. The seed germination rate is low and difficult to harvest. The survival rate of cutting propagation is high, and cutting propagation is the main production in forestry. In this paper, the cuttage experiment was carried out to study effects of different sources of cuttings and different substrate on the rooting rate of S. sempervirens. The results showed that the rooting rate was the highest when the ratio of perlite, vermiculite and sand was 1:1:1. The rooting rate of type II, III, V cuttings was higher, and the new shoots had strong sprouting ability and fast growth without obvious age effect and position effect.