Abstract:To explore the effects of different cultivation substrates on the growth of Polygonatum cyrtonema, the experiment of cultivation of P. cyrtonema under Phyllostachys edulis forest was carried out. Humus soil plus plant ash, humus soil plus organic fertilizer, humus soil plus fungus stick waste were used as the matrix materials were compared with humus soil. The results showed that the survival rate of P. cyrtonema in the matrix of four formulas was more than 95%, and the difference was not significant. Treatment is the best (humus: plant ash =95∶5). The average ground diameter, plant height, number of leaves (13.18 pieces) and fresh weight of root tuber of C. polyflorum were 0.69 cm, 74.46 cm, and 56.18 g. The second was humus soil and organic fertilizer (humus: organic fertilizer =90∶10), with ground diameter (0.66 cm), plant height (68.28 cm), number of leaves (12.98 pieces) and fresh weight of root tuber (53.38 g). Compared with the control group, the differences of plant ash and organic fertilizer reached extremely significant level (p-value < 0.01).Because the cost of plant ash is high and it is a non-renewable resource in a short time, more organic fertilizer should be used and less plant ash should be used in the substrates of the cultivation of Polygonatum Cyrtonema