Abstract:Abstract: In order to explore the effectiveness of biological agents on Hyphantria cunea, HycuNPV and BT were used in Dawu County,of Hubei Province. The results showed that the adjusted mortality rate was 26.71% after 7 days, 67.96% after 10 days and 99.88% after 13 days.The adjusted mortality rate of controlling third-generation Hyphantria cunea larvae administration with a single HycuNPV was 17.3% after 7 days ,40.67% after 10 days, 8 0.33% after 13 days, and 91.67% after 15 days.Combination of HycuNPV with BT had an adjusted mortality of controlling first-generation was 66% after 7 days,95.44% after 10 days, and 99.88% after 13 days.Combination of HycuNPV with BT had an adjusted mortality of controlling third-generation 43.33% after 7 days,76.67% after 10 days, 9 1.33%after 13 days, and 96% after 15 days.All indicate that the combination of HycuNPV and BT is faster and significantly faster than a single HycuNPV.The results of the first generation and the third generation of Hyphantria cunea larvae were consistent, but the first generation was better.The combination of HycuNPV and BT has obvious synergistic effect and can also significantly reduce the prevention and control cost.