猕猴桃远缘杂交胚胎挽救技术初探

study on Embryo Rescue of Distant Hybridization in Actinidia arguta

摘要:

为创新猕猴桃种质 , 以软枣猕猴桃栽培品种 6猕枣 2 号》为母本 , 以美味猕猴桃 6磨 山 3 号》为父本进行远缘杂 交 , 对杂交后的未成熟胚进行胚胎挽救 , 分析不同胚龄和培养基对胚胎挽救效果的影响 。结果显示:授粉 35d 的未成熟 胚在 ⑨号培养基中可同时诱导出丛生芽和胚性愈伤组织 , 且 二 者诱导率均为最高 , 分别为 10. 00%和 13. 33% 。 丛生芽 在添加 0. 5 mg ● L- 1 IBA的 1/2MS培养基上生根效果最佳 。通过杂种未成熟胚的胚胎抢救 , 成功获得远缘杂交后代 , 创制了猕猴桃新种质 , 为猕猴桃品种选育提供了基础 。

Abstract:

To innovate kiwifruit germplasm , the distant hybridization was cayyied out using cultivar 6Mizao No . 2》ofA. arguta as female parent and 6Moshan No . 3》of A. deliciosa as male parent. The embryo rescue was performed using hy- brid immature embryos , and the effects of different embryo age and medium on embryo rescue were analyzed. The results indicated that the immature embryos after 35days of pollination on ⑨ medium can induce multiple shoots and embryonic callus , and the induction rates of both were the highest , 10% and 13. 33% , respectively. The rooting effect of multiple shoots on 1/2MSmedium with 0. 5 mg ● L—1 IBA was better . Through the embryo rescue of immature hybrid embryos , the distant hybrid offspring were successfully obtained , and the new germplasm of kiwifruit was innovated , which provided the basis for kiwifruit breeding.