‘红颜’草莓组培快繁技术试验

摘要:

以‘红颜’草莓匍匐茎的茎尖为试材，经初代培养和继代培养获得脱毒苗。结果表明：初代愈伤诱导培养最适宜的培养基配方为MS+NAA 1.0 mg·L-1+6-BA 5 mg·L-1;最适宜愈伤增殖的培养基配方为MS+NAA 1 mg·L-1+6-BA 10 mg·L-1+TDZ 1 mg·L-1;最适宜诱导萌芽的培养基配方为MS+NAA 0.40 mg·L-1+6-BA 10 mg·L-1+活性炭1g·L-1。在继代培养中最适宜诱导愈伤的部位为叶片，最适宜诱导萌芽的部位为茎尖处；最适宜叶片诱导出愈伤组织的培养基配方为MS+NAA 0.50 mg·L-1+TDZ 20 mg·L-1+活性炭1g·L-1;最适宜茎尖诱导萌芽的培养基配方为MS+IBA 5 mg·L-1+活性炭3 g·L-1;最佳生根培养基配方为1/2MS+IBA 5 mg·L-1+活性炭3 g·L-1。

Abstract:

In this study, Fragaria×ananassa cv. ‘Benihopp' stolon tip was used as experimental material.After primary and successive culture, the virus-free seedlings were obtained.The results showed that the optimal medium formula for callus induction was MS+ NAA 1.0 mg·L-1 +6-BA 5 mg·L-1;the optimal medium formula for callus proliferation was MS+NAA 1 mg·L-1+6-BA 10 mg·L-1+TDZ 1 mg·L-1;the optimal medium formula for inducing germination was MS+NAA 0.40 mg·L-1+6-BA 10 mg·L-1+activated carbon 1 g·L-1;the most suitable site for callus induction was leaf; the most suitable site for germination was the shoot apex; the optimal medium formula for inducing callus from leaf tissue was MS+NAA 0.50 mg·L-1+TDZ 20 mg·L-1 + activated carbon 1 g·L-1;the optimal medium formula for inducing root growth from shoot apex was MS+IBA 5 mg·L-1 + activated carbon 3 g·L-1;the optimal medium formula for rooting was 1/2MS+IBA 5 mg·L-1+activated carbon 3 g·L-1.