Based on the data obtained by LiDAR system; this paper aims to explore and establish a model for estimating the stock of mixed tree species in Hubei Province. The study area covered 386 sample plots(small classes)in 9 cities and 17 counties and urban areas; involving three forest types(broadleaf forest; coniferous forest and coniferous mixed forest); which were divided into five vegetation areas; namely; Tongbai Mountain hills in Dabie Mountain; mountain hills in northwest Hubei Province; mountain hills in southeast Hubei Province; lake in Jianghan Plain and mountain land in southwest Hubei Province.The forest parameter characteristic variables were extracted from the point cloud data; and combined with the field survey data; the machine algorithm KNN; XGBoost and RF models were used to estimate the forest stock; and the determination coefficient was used to evaluate the estimation accuracy of the models; and the estimation results were compared and analyzed.The results show that: 1)the estimated value of RF model is close to the actual value; and the accuracy is higher than KNN and XGBoost model. 2)The estimation accuracy of forest types in different geomorphic regions was different; and the estimation accuracy of coniferous forest was higher than that of broad-leaved forest. The estimation accuracy was correlated with stand closure; stand age; origin and other factors. The estimation accuracy was higher when stand closure was higher. The estimation accuracy of middle age; near mature forest and over mature forest was higher. The accuracy of planted forest is higher than that of natural forest. 3)The accuracy of the estimated storage volume is correlated with the interval of the true value. When the true value tends to a certain interval of low value and high value; the estimation accuracy decreases.Through the inversion results of LiDAR data and the verification of ground survey data; the accuracy of the model is reflected; and the fusion application of forestry investigation and LiDAR is promoted. It is necessary to further compare various models and explore the relevant factors affecting the estimation accuracy among forest distribution; forest structure characteristics and stand factors.