血叶兰无菌培养快繁技术优化

Optimization of Aseptic Culture and Rapid Propagation Technique of Ludisia discolor

摘要:

血叶兰是珍稀濒危药用植物，具有巨大市场开发潜力，由于天然种群日趋缩减，亟需开展种质资源繁殖保护等研究。以血叶兰蒴果为外植体，表面消毒后切开，播种于培养基 2 号 1/2MS + NAA0.1 mg?L?1 + 白糖 30g?L?1 + 香蕉泥 50mg?L?1 + 马铃薯 25mg?L?1 外植体诱导，血叶兰种子正常萌发生长；血叶兰继代和生根培养基为培养基 6 号 1/2MS + BA 0.2 mg?L?1 + IBA0.3mg?L?1 + NAA 0.01mg?L?1 + 香蕉泥 50mg?L?1 + 马铃薯 25mg?L?1，采用一步法继代生根成苗。组培瓶苗移栽基质为腐熟松树皮 + 珍珠岩 + 泥炭。

Abstract:

Ludisia discolor is a rare and endangered medicinal plant with great market development potential. The natural population is decreasing day by day, so it is urgent to carry out the study on germplasm resources reproduction and protection.The capsule of L. discolor was used as explants, the surface was disinfected and cut, and the seeds were seeded on the 2nd induction medium of 1/2MS + NAA 0.1 mg·L?1 + sugar 30 g·L?1 + banana mud 50 mg·L?1 + potato mud 25 mg·L?1. The seeds germinated normally. The 6th subculture and rooting medium of L. discolor were 1/2MS + BA 0.2 mg·L?1 + IBA 0.3 mg·L?1 + NAA 0.01 mg·L?1 + banana mud 50 mg·L?1 + potato mud 25 mg·L?1, and the seedlings were derived by one - step method. The tissue - cultured bottle plantlets were transplanted to a substrate with pine bark + perlite + peat.